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KMID : 0380219960290020122
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Journal of Biochemistry and Molecular Biology
1996 Volume.29 No. 2 p.122 ~ p.126
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Sequence Verification of Synthetic Oligonucleotides by Exonuclease Digestion and Matrix Assisted Laser Desorption Ionization Mass Spectrometry
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Kim Jin-Sung
Jang Jung-Suk
Choi Jong-Soon
Chang Yoon-Seok
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Abstract
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A series of oligonucleotides were synthesized by automatic DNA synthesizer. The purity of crude products was checked and their molecular weights determined by matrix assisted laser desorption ionization mass spectrometry (MALDI-MS) with an accuracy of better than 0.05% deviation even without using an internal standard. This mass determining technology in combination with partial digestion of oligonucleotides by 5’- and 3’-exonuclease provides a straightforward and simple method to obtain sequence information of oligonucleotides. The extension of this technology to the sequencing of modified oligonucleotides and genomic DNA and RNA might become possible.
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KEYWORD
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digestion, exonuclease, matrix assisted laser desorption ionization mass spectrometry, oligonucleotides, sequencing
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